Abstract
Among various strategies for constructing customized transcription activator-like effector nucleases (TALENs), the Golden Gate assembly is the most widely used and most characterized method. The principle of Golden Gate assembly involves cycling reactions of digestion and ligation of multiple plasmids in a single tube, resulting in PCR-, fragmentation-, and purification-free concatemerization of DNA-binding repeats. Here, we describe the protocols for Golden Gate assembly-based TALEN construction using the Platinum Gate TALEN Kit, which allows generation of highly active Platinum TALENs.
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Sakuma, T., Yamamoto, T. (2016). Engineering Customized TALENs Using the Platinum Gate TALEN Kit. In: Kühn, R., Wurst, W., Wefers, B. (eds) TALENs. Methods in Molecular Biology, vol 1338. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2932-0_6
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DOI: https://doi.org/10.1007/978-1-4939-2932-0_6
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2931-3
Online ISBN: 978-1-4939-2932-0
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