Seed World

Agdia Commercializes ELISA for Testing Vip3A Corn Varieties

Agdia, a provider of easy-to-use test kits for agricultural diagnostics, commercializes a new ELISA assay for detection of the Vip3A protein expressed in transgenic corn, event MIR162.
The Vip3A ELISA was specifically developed and validated for trait confirmation testing of seed lots using either individual kernels or leaf material. According to the company, the assay is sensitive enough to detect 1 Vip3A kernel in 100 negative kernels (1 percent).
The Agdia Vip3A ELISA is a complete kit, including antibody-coated microtiter plates, enzyme conjugate, positive control and extraction/wash buffers. Consistent with other ELISA assays in Agdia’s trait testing portfolio, the Vip3A protocol was designed with high-throughput testing and ease-of-use in mind. Using a common extraction process and short incubation steps, the entire test is completed in less than two hours.
For more information about Agdia and the new ELISA assay, visit