b'TESTINGSoybean Cyst Nematode DNA Test, 20/20 Seed LabsBe wary of yellow patches in your soybean field. If causedMolecular Test for Phytophthora Root Rot Variant by soybean cyst nematodeDetection, AYOS Technologies(SCN), up to 30% yield lossPhytophthora root rot is one of the most prevalent diseases may have occurred beforeaffecting soybean crops. In North America alone, it causes these visible symptoms evenannual yield losses of nearly $500 million. The use of resistant appear. Although relativelysoybean varieties is recognized as the most effective method new in Canada, SCN posesof combating this disease. However, new variants of the a significant threat to ourpathogen can rapidly overcome resistance genes, so its growing soybean industry.essential to know which variety to choose, based on a precise Endemic in the U.S., SCNknowledge of the variants present in a given field.causes an estimated $1.5 billion in losses annually. NowFrom a simple field sample, the AYOS molecular test uses is the time to monitor for andgenetic markers to identify quickly and precisely which contain SCN spread. variants (pathotypes) of Phytophthora root rot are present in a growers field, so that proper resistant varieties can be used in Traditional SCN detectionthe following year to fight the disease.methods are limited in detecting different nematodeThis choice of a resistant variety will allow a producer life stages and work best incultivating 200 ha of soybeans to avoid yield losses averaging situations of known or high$30,000 each year. The technology will further eliminate infestation. 20/20 Seed Labsthe dependence on chemicals used in seed treatment as is the first commercial labselection of the proper resistant variety will protect the plant in Canada to offer a DNA- from the seedling stage to full maturity.based diagnostic test able to detect SCN in soil, plant roots and seeds. This testing method provides an absolute identification of SCN and detection of even low levels of egg, cyst and motile forms.Early detection and containment are key to minimizing current and future losses to SCN. This test provides a needed tool for Canadian seed growers and producers to avoid SCN transfer between fields with planting seed or machinery and to monitor the effectiveness of resistant varieties. Its applicability to root tissues is also helpful for breeders involved in developing SCN-resistant varieties.Photo: Qing Yu, AAFCJULY 2022 GERMINATION.CA 11'